Amplification of fragments of ARH1 from cDNA, genomic DNA, or BAC clones (probe 1) was carried out under the conditions shown in Table 1. Reaction mixes all contained 1X NH4 buffer (Bioline, London, United Kingdom), 0.2 mM dNTP, 0.5 mM MgCl2, 0.04 u/µl Biotaq polymerase (Bioline), 0.02 µg/µl each of forward primer (F) and reverse primer (R), and 0.4 ng/µl of DNA template. Cycling conditions were as follows: for cDNA templates, 94°C for 3 min, 30 cycles of 94°C for 30 s, 56°C for 30 min, and 72° for 1 min, then 72°C for 7 min; for exons 5 and 6 from genomic DNA templates, 94°C for 3 min, 28 cycles of 94°C for 30 min, 57°C for 30 s and 72°C for 1 min, then 72°C for 7 min; for exons 7, 8, and 9 from genomic DNA templates, 94°C for 3 min, 30 cycles of 94°C for 30 min, 57°C for 30 s and 72°C for 1 min, then 72°C for 7 min; for BAC clone templates, 94°C for 3 min, 29 cycles of 94°C for 30 s, 57°C for 30 s and 72° for 3 min, then 72°C for 7 min.