Supplemental Video 1
TRITC-conjugated HMW dextran (155 kDa) was infused through a carotid catheter in a B6 SCID recipient of a BALB/c skin graft. Graft vasculature was observed using intravital microscopy fitted with a red/green dual-wavelength filter. Vascular-flow dynamics can be observed in this video as the dextran is retained in the vessels and excluded from red blood cells.
Supplemental Video 2
HMW TRITC-conjugated dextran was infused through a carotid catheter in B6 recipients of a BALB/c skin graft either 4 days (a) or 5 days (b) after reconstitution with wild-type B6 splenocytes. (a) Four days after reconstitution, all vessels illuminate with the contrast and have characteristics of steady, undisturbed flow. (b) Five days after reconstitution, several vessels fail to illuminate with TRITC-dextran. As the video pans upward, sluggish flow can be seen in another vessel that does contain the contrast. Flow stasis can be seen at the junction of the nonilluminated and illuminated vessels.
Supplemental Video 3
TRITC-dextran contrast was infused in B6 SCID recipients of BALB/c skin grafts 4 days after reconstitution with wild-type B6 T cells. Animals then received either naive (a) or activated (b) B6 T cells labeled with CFSE and suspended in anti-mouse CD62L antibody. (a) Naive cells fail to engage rejecting vasculature after treatment with anti-CD62L antibody. (b) Activated T cell interactions are unaffected by anti-CD62L antibody.
Supplemental Video 4
Engrafted B6 SCID mice received infusions of activated T cells 4 days post-reconstitution. Animals received anti-CD62P (a); anti-CD62P and anti-CD62E (b); or isotype control (c). (a) A single cell adheres to the top venule as the recording begins. Most other cells fail to interact. Anti-CD62P alone blocks most rolling interactions, but only 50% of adhesion interactions. (b) Additional anti-CD62E blocks firm adhesion. Most cells pass through without interacting, though some residual rolling occurs. (c) Animals receiving isotype controls demonstrate significant rolling and firm adhesion interactions.